Knockdown of VGLL4 was achieved by transfecting HCTt116 cells with specific siRNA (Silencer® select Product # s229766, s229764). Immunofluorescence analysis was performed on HCT116 cells (untransfected, panel a-d), transfected with VGLL4 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703012, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to VGLL4 (green). The images were captured at 60X magnification.
Knockdown of VGLL4 was achieved by transfecting HCTt116 cells with specific siRNA (Silencer® select Product # s229766, s229764). Immunofluorescence analysis was performed on HCT116 cells (untransfected, panel a-d), transfected with VGLL4 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703012, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to VGLL4 (green). The images were captured at 60X magnification.
Supplier Page from Thermo Fisher Scientific for VGLL4 Antibody